Logo Leibniz Universität Hannover
Logo: Institute of Plant Nutrition/Leibniz Universität Hannover
Logo Leibniz Universität Hannover
Logo: Institute of Plant Nutrition/Leibniz Universität Hannover
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Äkta pure

The Äkta pur is a liquid chromatography system, which facilitates the purification of proteins from complex biological samples. The machine is equipped with a conductivity and a UV-VIS detector as well as a fraction collector to isolate fractions of the chromatography, which can then be analyzed by other methods.

CNS-Analyser

The CNS-Analyser is used for the quantification of carbon, nitrogen and sulphur in dried plant material or dried soil samples.

The weighed sample is burned in a combustion tube under oxygen supply. Helium is used as carrier gas. The combustion products (NOx, CO2, SOx) will be reduced in a reduction tube to defined gases (N2, CO2, SO2). Water and halogens will be removed by chemical adsorption. CO2 and SO2 will be intermediately bound on columns. The thermal conductivity detector of the machine will first quantify the N2 content. Then CO2 will be desorbed from the column by heat and measured. Eventually SO2 will be desorbed and measured.

Confocal Laser Scanning Mikroscope

The confocal microscope (Leica SP8, upright) allows the detection of fluorescent signals in biological samples. The fluorescence is elicited by laser light. Our microscope has four solid state lasers with the wavelengths 448. 488, 514, and 552 nm. The fluorescence light can be detected by two photomultiplier tubes and two high-sensitivity HyD detectors.

HPLC

The High Performance Liquid Chromatography (HPLC) allows the chromatographic separation of analytes in a complex mixture in a fluid stream over a chromatographic column. Our machine is equipped with a diode array detector (DAD) and a fluorescence detector for the identification and quantification of analytes.

HPLC-QQQ-MS

The high performance liquid chromatography (HPLC) coupled to a triple quadrupole mass spectrometer (Agilent 6460) allows the identification and high-sensitive quantification of metabolites due to their mass and the masses of their fragmentation products generated in a collision cell.

Stereo Fluorescence Microskope

The Nikon SMZ25 is a fluorescence stereo microscope with a high resolution at a zoom factor of 0.63x (entire 35 mm petri dish visible) up to 15.75x (25:1). With an automatic control of the z-axis precise z-image stacks can be generated, which can be integrated by the software to clear images with high depth of focus.