Publications Research Group Witte

  • Niehaus, M., Straube, H., Specht, A., Baccolini, C., Witte, C.-P., Herde, M. (2022): The nucleotide metabolome of germinating Arabidopsis thaliana seeds reveals a central role for thymidine phosphorylation in chloroplast developmentTHE PLANT CELL 2022, 1-24.
    DOI: 10.1093/plcell/koac207
  • Heinemann, K. J., Yang, S.-Y., Straube H., Medina-Escobar N., Varbanova-Herde M., Herde M., Rhee S., Witte C.-P. (2021): Initiation of cytosolic plant purine nucleotide catabolism involves a monospecific xanthosine monophosphate phosphataseNature Communications
    DOI: 10.1038/s41467-021-27152-4
  • Kim, S.-H., Witte, C.-P, Rhee, S. (2021): Structural basis for the substrate specificity and catalytic features of pseudouridine kinase from Arabidopsis thalianaNucleic Acids Research 49, 491–503.
    DOI: 10.1093/nar/gkaa1144
  • Rinne, J., Witte, C.-P. and Herde, M. (2021): Loss of MAR1 Function is a Marker for Co-Selection of CRISPR-Induced Mutations in PlantsFrontiers in Genome Editing, 09 August 2021
    DOI: 10.3389/fgeed.2021.723384
  • Straube, H., Witte, C.P., Herde, M. (2021): Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC–MS TechniquesCells 10, 689.
    DOI: 10.3390/cells10030689
  • Zhu et. al (u.a. Herde, M.) (2021): Calmodulin-like protein CML24 interacts with CAMTA2 and WRKY46 to regulate ALMT1-dependent Al resistance in Arabidopsis thalianaNew Phytologist 233, 2471–2487
    DOI: 10.1111/nph.17812
  • Chen, M., Witte, C.-P. (2020): A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate AccumulationThe Plant Cell 32, 722-739.
    DOI: 10.1105/tpc.19.00639
  • Durian G, Sedaghatmehr M, Matallana-Ramirez L P, Schilling S, Schaepe S, Guerra T, Herde M, Witte C-P, Müller-Röber B, Schulze W X, Balazadeh S, Romeis T (2020): Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1Plant Cell 32, 1610-1625.
    DOI: 10.1105/tpc.19.00810
  • Niehaus, M., Straube, H., Künzler, P., Rugen, N., Hegermann, J., Giavalisco, P., Eubel, H., Witte, C.-P., Herde, M. (2020): Rapid affinity purification of tagged plant mitochondria (Mito-AP) for metabolome and proteome analysesPlant Physiology 182, 1194–1210.
    DOI: 10.1104/pp.19.00736
  • Safavi-Rizi, V., Herde, M. and Stöhr, C. (2020): RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato rootScientific Reports 10, 1-17.
  • Straube, H., Niehaus, M., Zwittian, S., Witte, C.-P., Herde, M. (2020): Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolismThe Plant Cell 33, 270–289.
    DOI: 10.1093/plcell/koaa028
  • Witte, C.-P. and Herde, M. (2020): Nucleotide metabolism in plantsPlant Physiology 182, 63–78.
    DOI: 10.1104/pp.19.00955
  • Baccolini, C., Witte, C.P. (2019): AMP and GMP Catabolism in Arabidopsis Converge on Xanthosine, which Is Degraded by a Nucleoside Hydrolase HeterocomplexThe Plant Cell 31, 734–751.
    DOI: 10.1105/tpc.18.00899
  • Kang, P.-A., Oh, J., Lee, H., Witte, C.-P., Rhee, S. (2019): Crystal structure and mutational analyses of ribokinase from Arabidopsis thalianaJournal of Structural Biology 206, 110-118.
    DOI: 10.1016/j.jsb.2019.02.007
  • Liu, B., Winkler, F., Herde, M., Witte, C.-P., Großhans, J. (2019): A link between deoxyribonucleotide metabolites and embryonic cell cycle controlCurrent Biology 29, 1187-1192.
    DOI: 10.1016/j.cub.2019.02.021
  • Chen, M., Urs, M.J., Sánchez-González, I., Olayioye, M.A., Herde, M., Witte C.-P. (2018): m6A RNA Degradation Products are Catabolized by an Evolutionarily Conserved N6-methyl-AMP Deaminase in Plant and Mammalian CellsThe Plant Cell 30, 1511–1522.
    DOI: 10.1105/tpc.18.00236
  • Kirchner, T.W., Niehaus, M., Rössig, K.L., Lauterbach, T., Herde, M., Küster, H. and Schenk , M.K. (2018): Molecular background of Pi deficiency-induced root hair growth in Brassica carinata – A fasciclin-like arabinogalactan protein is involvedFrontiers in Plant Science 9, 1372.
    DOI: 10.3389/fpls.2018.01372
  • Schroeder, R.Y., Zhu, A., Eubel, H., Dahncke, K., Witte, C.-P. (2018): The ribokinases of Arabidopsis thaliana and Saccharomyces cerevisiae are required for ribose recycling from nucleotide catabolism which in plants is not essential to survive prolonged dark stressNew Phytologist 217, 233-244.
    DOI: 10.1111/nph.14782
  • Kirchner, T.W., Niehaus, M., Debener, T., Schenk, M.K., Herde, M. (2017): Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinataPLOS ONE 12, 0185429.
    DOI: 10.1371/journal.pone.0185429
  • Myrach, T., Zhu, A., Witte, C.-P. (2017): The assembly of the plant urease activation complex and the essential role of the urease accessory protein G (UreG) in delivery of nickel to ureaseThe Journal of Biological Chemistry 292, 14556-14565.
    DOI: 10.1074/jbc.M117.780403
  • Yang, Z.-B., He, C., Ma, Y., Herde, M., Ding, Z. (2017): Jasmonic acid enhances Al-induced root-growth inhibitionPlant Physiology 173, 1420–1433.
    DOI: 10.1104/pp.16.01756
  • Yilamujiang, A., Zhu, A., Ligabue-Braun, R., Bartram, S., Witte, C.-P., Hedrich, R., Hasabe, M., Schöner, C.R., Schöner, M.G., Kerth, G., Carlini, C.R. & Mithöfer, A. (2017): Coprophagous features in carnivorous Nepenthes plants: a task for ureasesScientific Reports 7, 11647.
    DOI: 10.1038/s41598-017-11999-z
  • Chen, M., Herde, M., Witte, C.P. (2016): Of the nine cytidine deaminase like genes in Arabidopsis thaliana eight are pseudogenes and only one is required to maintain pyrimidine homeostasis in vivoPlant Physiology 171, 799-809.
    DOI: 10.1104/pp.15.02031
  • Matschi, S., Hake, K., Herde, M., Hause, B. and Romeis, T. (2015): The Calcium-Dependent Protein Kinase CPK28 Regulates Development by Inducing Growth Phase-Specific, Spatially Restricted Alterations in Jasmonic Acid Levels Independent of Defense Responses in ArabidopsisThe Plant Cell 27, 591-606.
    DOI: 10.1105/tpc.15.00024​​
  • Hauck, O.K., Scharnberg, J., Medina Escobar, N., Wanner, G., Giavalisco, P., Witte, C.P. (2014): Uric acid accumulation in an Arabidopsis urate oxidase mutant impairs seedling establishment by blocking peroxisome maintenanceThe Plant Cell 26, 3090-3100.
    DOI: 10.1105/tpc.114.124008
  • Herde, M., Howe G.A. (2014): Host plant-specific remodeling of midgut physiology in the generalist insect herbivore Trichoplusia niInsect Biochemistry and Molecular Biology 50, 58-67.
    DOI: 10.1016/j.ibmb.2014.03.013
  • Romeis, T., Herde, M. (2014): From local to global: CDPKs in systemic defense signaling upon microbial and herbivore attackCurrent Opinion in Plant Biology 20, 1-10.
    DOI: 10.1016/j.pbi.2014.03.002
  • Aragüez I, Cruz-Rus E, Botella MÁ, Medina-Escobar N, Valpuesta V. (2013): Proteomic analysis of strawberry achenes reveals active synthesis and recycling of L-ascorbic acidJournal of Proteomics 27, 160-79.
    DOI: 10.1016/j.jprot.2013.03.016
  • Aragüez, I., Osorio, S., Hoffmann, T., Rambla, J.L., Medina-Escobar, N., Granell, A., Botella, M.Á., Schwab, W., Valpuesta, V. (2013): Eugenol production in achenes and receptacles of strawberry fruits is catalyzed by synthases exhibiting distinct kineticsPlant Physiology 163, 946-958.
    DOI: 10.1104/pp.113.224352
  • Chuang, W.-P., Herde, M., Ray, S., Castano-Duque, L., Howe, G.A., Luthe, D.S. (2013): Caterpillar attack triggers accumulation of the toxic maize protein RIP2New Phytologist 201, 928-939.
    DOI: 10.1111/nph.12581
  • Dahncke, K., Witte, C.P. (2013): Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in ArabidopsisThe Plant Cell 25, 4101-4109.
    DOI: 10.1105/tpc.113.117184
  • Dubiella, U., Seybold, H., Durian, G., Komander, E., Lassig, R., Witte, C.P., Schulze, W.X., Romeis, T. (2013): Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagationProceedings of the National Academy of Sciences of the United States of America 110, 8744-8749.
    DOI: 10.1073/pnas.1221294110
  • Herde, M., Koo, A.J.K., Howe, G.A. (2013): Elicitation of jasmonate-mediated defense responses by mechanical wounding and insect herbivoryMethods in Molecular Biology 1011, 51-61.
    DOI: 10.1007/978-1-62703-414-2_5
  • Merchante, C., Vallarino, J.G., Osorio, S., Aragüez, I., Villarreal, N., Ariza, M.T., Martínez, G.A., Medina-Escobar, N., Civello, M.P., Fernie, A.R., Botella, M.A., Valpuesta, V. (2013): Ethylene is involved in strawberry fruit ripening in an organ-specific mannerJournal of Experimental Botany 64, 4421-4439.
    DOI: 10.1093/jxb/ert257
  • Nakata, M., Mitsuda, N.,Herde, M., Koo, A.J.K., Moreno, J.E., Suzuki, K., Howe, G.A., Ohme-Takagi, M. (2013): A bHLH-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, acts as a repressor to negatively regulate jasmonate signaling in ArabidopsisThe Plant Cell 25, 1641-1656.
    DOI: 10.1105/tpc.113.111112
  • Schulz, P., Herde, M., Romeis, T. (2013): Calcium-Dependent Protein Kinases: Hubs in plant stress signaling and developmentPlant Physiology 163, 523-530.
    DOI: 10.1104/pp.113.222539
  • Werner, A.K., Medina-Escobar, N., Zulawski, M., Sparkes, I.A., Cao, F.Q., Witte, C.P. (2013): The ureide degrading reactions of purine ring catabolism employ one aminohydrolase and three amidohydrolases in Arabidopsis, soybean and ricePlant Physiology 163, 672-681.
    DOI: 10.1104/pp.113.224261
  • Osorio, S., Bombarely, A., Giavalisco, P., Usadel, B., Stephens, C., Aragüez, I., Medina-Escobar, N., Botella, M.A., Fernie, A.R., Valpuesta, V. (2011): Demethylation of oligogalacturonides by FaPE1 in the fruits of the wild strawberry Fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruitJournal of Experimental Botany 62, 2855-2873.
    DOI: 10.1093/jxb/erq465
  • Rietz S, Stamm A, Malonek S, Wagner S, Becker D, Medina-Escobar N, Corina Vlot A, Feys BJ, Niefind K, Parker JE. (2011): Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunityNew Phytologist 191, 107-119.
    DOI: 10.1111/j.1469-8137.2011.03675.x
  • Werner, A.K., Witte, C.P. (2011): The biochemistry of nitrogen mobilization: purine ring catabolismTrends in Plant Science 16, 381-387.
    DOI: 10.1016/j.tplants.2011.03.012
  • Witte, C.P. (2011): Urea metabolism in plantsPlant Science 180, 431-438.
    DOI: 10.1016/j.plantsci.2010.11.010
  • Cao, F.Q., Werner, A.K., Dahncke, K., Romeis, T., Liu, L.H., Witte, C.P. (2010): Identification and characterization of proteins involved in rice urea and arginine catabolismPlant Physiology 154, 98-108.
    DOI: 10.1104/pp.110.160929
  • Lee, S., Badieyan, S., Bevan, D., Herde, M., Gatz, C., Tholl, D. (2010): Herbivore-induced and floral homoterpene volatiles are biosynthesized by a single CytP450 enzyme (CYP82G1) in Arabidopsis Proceedings of the National Academy of Sciences of the United States of America 107, 21205-21210.
    DOI: 10.1073/pnas.1009975107
  • Muñoz, C., Hoffmann, T., Medina Escobar, N., Ludemann, F., Botella, M.A., Valpuesta, V., Schwab, W. (2010): The strawberry fruit Fra a allergen functions in flavonoid biosynthesisMolecular Plant 3, 113-124.
    DOI: 10.1093/mp/ssp087
  • Werner, A.K., Romeis, T., Witte, C.P. (2010): Ureide catabolism in Arabidopsis thaliana and Escherichia coli Nature Chemical Biology 6, 19-21.
    DOI: 10.1038/nchembio.265
  • Witte, C.P, Dubiella, U., Seal, A., Zappel, N., Demoulière, R., Romeis, T. (2010): Tobacco calcium-dependent protein kinases are differentially phosphorylated in vivo as part of a kinase cascade that regulates stress responseJournal of Biological Chemistry 285, 9740-9748.
    DOI: 10.1074/jbc.M109.052126
  • Herde, M., Gärtner, K., Köllner, T.G., Fode, B., Boland, W., Gershenzon, J., Gatz, C., Tholl, D. (2008): Identification and regulation of TPS04/GES, an Arabidopsis geranyllinalool synthase catalyzing the first step in the formation of the insect-induced volatile C16-homoterpene TMTTThe Plant Cell 20, 1152-1168.
    DOI: 10.1105/tpc.106.049478
  • Osorio, S., Castillejo, C., Quesada, M., Medina-Escobar, N., Brownsey, G., Suau, R., Heredia, A., Botella, M.A., Valpuesta, V. (2008): Partial demethylation of oligogalacturonides by Pectin Methyl Esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca)The Plant Journal 54, 43-55.
    DOI: 10.1111/j.1365-313X.2007.03398.x
  • Werner, A.K., Sparkes, I.A., Romeis, T., Witte, C.P. (2008): Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybeanPlant Physiology 146, 418-430.
    DOI: 10.1104/pp.107.110809
  • Noël, L.D., Cagna, G., Stuttmann, J., Wirthmüller, L., Betsuyaku, S., Witte, C.P., Colby, T. and Parker, J.E. (2007): Interaction between SGT1 and cytosolic/nuclear HSC70 chaperones regulates Arabidopsis immune responsesPlant Cell 19, 4061-4076.
  • Iannetta, P.P.M., Laarhoven, L.-J., Medina-Escobar, N., James, E.K., McManus, M.T., Davies H.V., Harren, F.J.M. (2006): Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruitPhysiologia Plantarum 127, 247–259.
  • Lopez, A.P., Portales, R.B., López-Ráez, J.A., Medina-Escobar, N., Blanco, J.M., Franco, A.R. (2006): Characterization of a strawberry late-expressed and fruit-specific peptide methionine sulphoxide reductase Physiologia Plantarum 126, 129–139.
  • Böhmer, M., Kurth, J., Witte, C.P., Romeis, T. (2005): Function of plant Calcium-Dependent Protein Kinases in the activation of abiotic and pathogen-related stress responses and its potential application in the generation of stress-resistant plantsFloriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues (1st Edition), J.A. Teixeira da Silva, ed (New Dehli):Regency Publications.
  • Feys, B.J., Wiermer, M., Bhat, R.A., Moisan, L.J., Medina-Escobar, N., Neu, C., Cabral, A., Parker, J.E. (2005): Arabidopsis SENESCENCE-ASSOCIATED GENE101 Stabilizes and Signals within an ENHANCED DISEASE SUSCEPTIBILITY1 Complex in Plant Innate ImmunityPlant Cell 17, 2601-2613.
  • Witte, C.P., Rosso, M.G., Romeis, T. (2005): Identification of three urease accessory proteins that are required for urease activation in Arabidopsis thalianaPlant Physiology 139, 1155-1162.
  • Witte, C.P., Tiller, S., Isidore, E., Davies, H.V., Taylor, M.A. (2005): Analysis of two alleles of the urease gene from potato: polymorphisms, expression and extensive alternative splicing of the corresponding mRNA Journal of Experimental Botany 56, 91-99.
  • Iannetta, P.P., Medina Escobar, N., Ross, H.A., Souleyre, E.J.F., Hancock, R.D., Witte, C.P., Davies, H.V. (2004): Identification, cloning and expression analysis of strawberry (Fragaria ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenasePhysiologia Plantarum 121, 15-26.
  • Witte, C.P., Noel, L.D., Gielbert, J., Parker, J.E., Romeis, T. (2004): Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitopePlant Molecular Biology 55, 135-147.
  • Ataya, F.S., Witte, C.P., Galvan, A., Igeno, M.I., Fernandez, E. (2003): Mcp1 encodes the molybdenum cofactor carrier protein in Chlamydomonas reinhardtii and participates in protection, binding, and storage functions of the cofactorJournal of Biological Chemistry 278, 10885-10890.
  • Glinski, M., Romeis, T., Witte, C.P., Wienkoop, S., Weckwerth, W. (2003): Stable isotope labeling of phosphopeptides for multiparallel kinase target analysis and identification of phosphorylation sitesRapid Communications in Mass Spectrometry 17, 1579-1584.
  • Medina-Escobar, N., Haupt, S., Thow, G., Boevink, P., Chapman, S., Oparka, K. (2003): High throughput viral expression of cDNA-GFP fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmataPlant Cell 15, 1507-1523.
  • Bacanamwo, M., Freyermuth, S.K., Palacios, J.M., Witte, C.P., Polacco, J.C. (2002): Nitrification, denitrification and the N cycleFinan, T.M., O´Brian, M.R., Layzell, D.B., Vessey, J.K., Newton, W., eds., Nitrogen Fixation: Global Perspectives. CABI Publishing, Hamilton, Ontario, UK, pp 314-318.
  • Bacanamwo, M., Witte, C.P., Lubbers, M.W., Polacco, J.C. (2002): Activation of the urease of Schizosaccharomyces pombe by the UreF accessory protein from soybeanMolecular Genetics and Genomics 268, 525-534.
  • Blanco-Portales R, Medina-Escobar N, Lopez-Raez JA, Gonzalez-Reyes JA, Villalba JM, Moyano E, Caballero JL, Munoz-Blanco J. (2002): Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragaria x ananassa cv. Chandler) Journal of Experimental Botany 53, 1723-1734.
  • Brown, J.W., Simpson, C.G., Thow, G., Clark, G.P., Jennings, S.N., Medina-Escobar, N., Haupt, S., Chapman, S.C., Oparka, K.J. (2002): Splicing signals and factors in plant intron removalBiochemical Society Transactions 30, 146-149.
    DOI: 10.1042/bst0300146
  • Witte, C.P., Tiller, S.A., Taylor, M.A., Davies, H.V. (2002): Leaf urea metabolism in potato. Urease activity profile and patterns of recovery and distribution of N-15 after foliar urea application in wild-type and urease-antisense transgenics Plant Physiology 128, 1129-1136.
  • Redondo-Nevado J., Moyano E., Medina-Escobar N., Caballero J.L., Munoz-Blanco J. (2001): A fruit-specific and developmentally regulated endopolygalacturonase gene from strawberry (Fragaria x ananassa cv. Chandler)Journal of Experimental Botany 52, 1941-1945.
  • Witte, C.-P., Isidore, E., Tiller, S.A., Davies, H.V., Taylor, M.A. (2001): Functional characterisation of urease accessory protein G (ureG) from potato Plant Molecular Biology 45, 169-179.
  • Witte, C.-P., Medina-Escobar, N. (2001): In-gel detection of urease with nitroblue tetrazolium and quantification of the enzyme from different crop plants using the indophenol reaction Analytical Biochemistry 290, 102-107.
  • Witte, C.-P., Tiller, S.A., Taylor, M.A., Davies, H.V. (2001): Addition of nickel to Murashige & Skoog medium in plant tissue culture activates urease and may reduce metabolic stressPlant Cell, Tissue and Organ Culture 68, 103-104.
  • Witte, C.P., Hien Le, Q., Bureau, T., Kumar, A. (2001): Terminal-repeat retrotransposons in miniature (TRIM) are involved in restructuring plant genomesProceedings of the National Academy of Sciences of the United States of America, 13778-13783.
  • Medina-Escobar, N., Cardenas, J., Munoz-Blanco, J., Caballero, J.L. (1998): Cloning and molecular characterization of a strawberry fruit ripening-related cDNA corresponding a mRNA for a low-molecular-weight heat-shock proteinPlant Molecular Biology 36, 33-42.
  • Moyano, E., Portero-Robles, I., Medina-Escobar, N., Valpuesta, V., Munoz-Blanco, J., Caballero, J.L. (1998): A fruit-specific putative dihydroflavonol 4-reductase gene is differentially expressed in strawberry during the ripening processPlant Physiology 117, 711-716.
  • Witte, C.-P., Blasco, R., Castillo, F. (1998): Microbial photodegradation of aminoarenes. Metabolism of 2-amino-4-nitrophenol by Rhodobacter capsulatus Applied Biochemistry and Biotechnology 69, 192-202.
  • Witte, C.-P., Igeño, M.I., Mendel, R., Schwarz, G., Fernández, E. (1998): The Chalmydomonas reinhardtii MoCo Carrier Protein is multimeric and stablilizes molybdopterin cofactor in a molybdate charged form FEBS Letters 431, 205-209.
  • Medina-Escobar N., Cardenas J., Valpuesta V., Munoz-Blanco J., Caballero J.L. (1997): Cloning and characterization of cDNAs from genes differentially expressed during the strawberry fruit ripening process by a MAST-PCR-SBDS method Analytical Biochemistry 248, 288-296.
  • Medina-Escobar, N., Cardenas, J., Moyano, E., Caballero, J.L., Munoz-Blanco, J. (1997): Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plantsPlant Molecular Biology 34, 867-877.
  • Caballero, J.L., Medina-Escobar, N., Cardenas, J., Munoz-Blanco, J. (1995): An easy non-radiactive procedure to check the quality of the ligation steps during the construction of cDNA librariesTrends in Genetics 12, 501-502.